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Preparation of nanoparticles and their use as contrast agents for in vivo imaging.
Odehnalová, Nikola ; Kratochvílová, Irena (referee) ; Turánek,, Jaroslav (advisor)
This diploma thesis deals with the optimalization of synthesis of gold nanoparticles and their surface modification allowing their use as contrast agents for in vivo imaging by CT. Gold nanoparticles were prepared by the Turkevich method and characterized by TEM, DLS, MADLS and UV -Vis. Their surface was functionalized with polyethylene glycol containing a thiol group forming a bond with the Au atoms in the surface of gold nanoparticles. The terminal end of the polymer was methylated or containing an aminooxy group forming an orthogonal bond with hyaluronic acid using click-chemistry. The eligibility for in vivo application of the prepared nanoparticles was verified with stability and cytotoxicity tests. The nanoparticles modified by methylated polyethyleneglycol were injected intravenously into a mouse and their application potential as contrast agents were verified by CT.
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The Introduction of Contact Ecotoxicity Tests for the Assessment of Terrestrial Ecosystems
Modlitbová, Pavlína ; Kráčmar, Stanislav (referee) ; Beklová, Miroslava (referee) ; Demnerová, Kateřina (referee) ; Vávrová, Milada (advisor)
This dissertation thesis is focus on using toxicity tests with testing organism - terrestrial isopod Porcellio scaber. This organism is use for toxicity assessment of selected anorganic compunds which could contaminate terrestrial ecosystem. This organism was selected for several reasons - sophisticated test methodology; well known biology of these animals and their ecological relevance. Besides classical endpoints such as mortality, change in mass of test specimens and the effect of the presence and concentration of contaminants onto food consumption; we monitored changes in the morphometric characteristics at the level of tissues and cells, the cytotoxicity and possibly bioaccumulation in various parts of the body of the organism. Selected test substance in this thesis were nanoparticles and inorganic salts. Wherein for chosen gold nanoparticles was carried out a complete study presents several levels of organization of living matter (organism, tissue, cell). For selected salts compound, this study was carried out on the behavior of organisms and classical endpoints.
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Preparation of nanoparticles and their use as contrast agents for in vivo imaging.
Odehnalová, Nikola ; Kratochvílová, Irena (referee) ; Turánek,, Jaroslav (advisor)
This diploma thesis deals with the optimalization of synthesis of gold nanoparticles and their surface modification allowing their use as contrast agents for in vivo imaging by CT. Gold nanoparticles were prepared by the Turkevich method and characterized by TEM, DLS, MADLS and UV -Vis. Their surface was functionalized with polyethylene glycol containing a thiol group forming a bond with the Au atoms in the surface of gold nanoparticles. The terminal end of the polymer was methylated or containing an aminooxy group forming an orthogonal bond with hyaluronic acid using click-chemistry. The eligibility for in vivo application of the prepared nanoparticles was verified with stability and cytotoxicity tests. The nanoparticles modified by methylated polyethyleneglycol were injected intravenously into a mouse and their application potential as contrast agents were verified by CT.
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The Introduction of Contact Ecotoxicity Tests for the Assessment of Terrestrial Ecosystems
Modlitbová, Pavlína ; Kráčmar, Stanislav (referee) ; Beklová, Miroslava (referee) ; Demnerová, Kateřina (referee) ; Vávrová, Milada (advisor)
This dissertation thesis is focus on using toxicity tests with testing organism - terrestrial isopod Porcellio scaber. This organism is use for toxicity assessment of selected anorganic compunds which could contaminate terrestrial ecosystem. This organism was selected for several reasons - sophisticated test methodology; well known biology of these animals and their ecological relevance. Besides classical endpoints such as mortality, change in mass of test specimens and the effect of the presence and concentration of contaminants onto food consumption; we monitored changes in the morphometric characteristics at the level of tissues and cells, the cytotoxicity and possibly bioaccumulation in various parts of the body of the organism. Selected test substance in this thesis were nanoparticles and inorganic salts. Wherein for chosen gold nanoparticles was carried out a complete study presents several levels of organization of living matter (organism, tissue, cell). For selected salts compound, this study was carried out on the behavior of organisms and classical endpoints.
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